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The human genome project led to the discovery that much of the genome consists of repeated sequences of nucleotides.These are thought to


A) have no function.
B) function as protection on the ends of DNA and as an attachment site on the centromere.
C) function in DNA replication.
D) produce rRNA.
E) function as a protection against cancer.

F) A) and B)
G) A) and C)

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PCR is often used in forensic analysis since


A) it requires no specialized equipment.
B) untrained personnel can carry out the procedure.
C) very small samples are frequently all that are available.
D) juries always trust the method.
E) it has been proven to determine the criminal.

F) None of the above
G) A) and B)

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The two techniques typically used to create transgenic animals are


A) microinjection of eggs and vortex mixing of eggs.
B) microinjection of eggs and electroshock of eggs.
C) electroshock of protoplasts and electroshock of eggs.
D) microinjection of protoplasts and electroshock of eggs.
E) microinjection of eggs and vortex mixing of protoplasts.

F) B) and E)
G) A) and E)

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Which of the following is(are) sites of in vivo gene therapy?


A) muscle cells
B) bone marrow
C) endothelium
D) liver
E) skin

F) A) and E)
G) B) and E)

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What is the substance required to cleave the vector DNA during recombinant DNA technology?


A) restriction enzymes
B) DNA ligase
C) plasmids
D) vectors
E) DNA helicase

F) A) and B)
G) A) and E)

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Why are genetically modified crop plants (GMOs) are able to express new traits?


A) They now contain genes from another organism.
B) They grow bacteria on the surface of leaves or roots.
C) There is no natural method for processes to be degraded in the organism.
D) Their normally occurring processes have been modified to serve different functions.
E) They have not been genetically modified enough to change the plant's genetics.

F) A) and B)
G) A) and D)

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Which of the following is NOT used in the production of recombinant DNA (rDNA) ?


A) vectors
B) restriction enzymes
C) DNA ligase
D) RNA
E) plasmids

F) B) and C)
G) A) and E)

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Which of the following is a site of ex vivo gene therapy?


A) bone marrow
B) muscle
C) blood
D) lungs
E) brain

F) A) and E)
G) A) and D)

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Which of the following is NOT correct regarding restriction enzymes?


A) They exist in bacteria to restrict the growth of viruses.
B) They cut double-stranded DNA at specific sites.
C) They are used during the manufacture of proteins.
D) They produce "sticky ends" that can bind foreign DNA.
E) They facilitate inserting foreign DNA into vector DNA.

F) C) and D)
G) None of the above

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Which of the following procedures would most likely be used to identify a specific individual of a population?


A) DNA cloning
B) transgenic DNA
C) polymerase chain reaction
D) DNA fingerprinting
E) genetic engineering

F) B) and D)
G) A) and B)

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In producing transgenic plants,cells called protoplasts are often used.Protoplasts are plant cells from which the cell wall has been removed.Why would the cell wall need to be removed?


A) The foreign DNA enters the cell through self-healing holes in the membrane but it could not pass through the cell wall.
B) The cell wall prevents the transgenic cell from growing into a mature plant.
C) The cell wall makes it difficult for the biotechnician to determine if the foreign DNA has been incorporated into the cell's genome.
D) The cell wall prevents the microinjection of foreign DNA into the cell because the needle cannot pass through it.
E) The foreign DNA passes through the plasmodesmata of the plant cell,which is blocked by the cell wall.

F) C) and D)
G) B) and E)

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Which of the following is not part of the process for PCR (polymerase chain reaction) ?


A) the use of an engineered DNA polymerase
B) the denaturing of the double-stranded DNA to yield single-strand DNA
C) multiple cycles of the chain reaction to yield a greater number of DNA copies
D) final product is single-stranded DNA
E) All of the answer choices are parts of the process for PCR.

F) A) and B)
G) A) and C)

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What structure is used to seal the DNA into an opening created by the restriction enzyme during recombinant DNA technology?


A) DNA ligase
B) restriction enzymes
C) plamids
D) vectors
E) DNA helicase

F) B) and E)
G) C) and D)

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The study of comparative genomics has given researchers the ability to look at multiple genomes from different species,which has led to new ideas about the evolutionary history of organisms.The conclusion is that


A) modern vertebrate species all evolved from very different ancestral species,so there is little similarity between their genomes.
B) modern vertebrate species all evolved from the same ancestral species,but they have changes so that their genomes are very different in modern times.
C) ancestral species contained very similar genomes,but modern vertebrate species have significant differences in their genomes.
D) modern vertebrate species evolved from the same ancestors and a large portion of their genome is conserved.
E) neither the ancestral species nor the modern one resemble each other genetically.

F) A) and B)
G) B) and C)

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The genome of Homo sapiens and Mus musculus are approximately the same size and composed of the same number of genes.

A) True
B) False

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Transposable elements make up 44% of the human genome.What is thought to be their function?


A) They fight cancer.
B) They code for rRNA.
C) They determine gender.
D) They help in the replication of DNA.
E) We are not sure of their function.

F) D) and E)
G) All of the above

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