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Prokaryotes regulate gene expression with a promoter.

A) True
B) False

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In eukaryotes, one gene may specify the construction of several proteins.

A) True
B) False

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What chemical force must be overcome in order to separate the two DNA strands during replication?


A) hydrophobic interactions
B) intrastrand folding
C) ionic bonds
D) phosphodiester bonds
E) hydrogen bonds DNA helicase helps to disrupt the hydrogen bonds between base pairs that hold the two strands together.

F) B) and E)
G) C) and D)

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Failure to remove introns from a primary mRNA would most likely cause


A) irregularities in transcription.
B) abnormal translation.
C) DNA to mutate.
D) DNA replication to fail.
E) transcription to terminate early. The exons of a eukaryotic primary mRNA contain the protein coding region of the gene, and thus the introns must be removed during primary mRNA splicing so that a usable message is obtained. If they are not removed, the protein that would result from translating the mRNA will be altered.

F) None of the above
G) B) and C)

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Which of the following events in gene expression control does not occur in the nucleus?


A) chromatin condensation
B) mRNA translation
C) DNA transcription
D) mRNA processing
E) transcription factor binding

F) A) and B)
G) B) and D)

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The three steps that are required for DNA replication are


A) unwinding, complementary base pairing, and joining.
B) base doubling, unwinding, and joining.
C) complementary base pairing, transcription, and translation.
D) unwinding, transcription, and translation.
E) unwinding, base doubling, and base pairing. In DNA replication, the DNA is first unwound by helicase. Then, new complementary nucleotides are positioned by complementary base pairing to each strand. The new nucleotides are then joined into a new strand.

F) B) and D)
G) B) and C)

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During the elongation cycle of translation, the A site on a ribosome functions in


A) receiving a new tRNA with the correct amino acid.
B) holding a polypeptide as amino acids are added.
C) attaching the small subunits of rRNA to the large subunit.
D) releasing the completed polypeptide.
E) processing the rRNA. During the elongation cycle, an incoming tRNA carrying the specified amino acid binds to the anticodon of the mRNA at the A site of the ribosome.

F) D) and E)
G) A) and B)

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Which of the following enzymes is needed to reseal breaks in the sugar-phosphate backbone during replication?


A) DNA polymerase
B) RNA polymerase
C) RNA ligase
D) helicase
E) DNA ligase DNA ligase reseals breaks in the sugar-phosphate backbone of DNA.

F) B) and E)
G) A) and D)

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Prokaryotes cannot regulate gene expression at which of the following levels?


A) mRNA transcription and mRNA translation
B) mRNA translation
C) alternative mRNA processing and chromatin compaction
D) mRNA translation and longevity of mRNA
E) mRNA transcription and longevity of mRNA

F) A) and E)
G) A) and C)

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Heterochromatin differs from euchromatin in that heterochromatin


A) is transcriptionally active, whereas euchromatin is not.
B) is not found in Barr bodies.
C) is transcriptionally inactive.
D) is the result of the unpacking of euchromatin.
E) contains DNA with accessible promoters. Heterochromatin is highly condensed chromatin that is transcriptionally inactive, because promoters are inaccessible to RNA polymerase. When it is unpacked, it becomes euchromatin.

F) All of the above
G) A) and E)

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RNA differs from DNA because RNA


A) is double-stranded, whereas DNA is not.
B) may double back and base pair with itself, whereas DNA does not.
C) is never single-stranded, whereas DNA may or may not be.
D) can self-replicate, whereas DNA replication requires enzymes.
E) is replicated along with DNA but does not require strand separation like DNA does.

F) C) and E)
G) A) and C)

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