Question 1

Which of the following is essential for the development of discrete,isolated colonies?

Accepted Answer

A)  broth medium
B)  differential medium
C)  selective medium
D)  solid medium
E)  assay mediumF) C) and E)
G) B) and C)

Question 2

Which type of microscope bombards a whole,metal-coated specimen with electrons moving back and forth over it?

Accepted Answer

A)  fluorescence
B)  differential interference contrast
C)  scanning electron
D)  transmission electron
E)  phase-contrastF) B) and C)
G) C) and D)

Question 3

Which type of microscope achieves the greatest resolution and highest magnification?

Accepted Answer

A)  bright-field
B)  dark-field
C)  phase-contrast
D)  fluorescence
E)  electronF) None of the above
G) D) and E)

Question 4

Which of the following magnifies the specimen to produce a real image of the specimen?

Accepted Answer

A)  condenser
B)  objective lens
C)  ocular lens
D)  body
E)  nosepieceF) A) and E)
G) None of the above

Question 5

Agar is an important component of media because

Accepted Answer

A)  bacteria require agar to grow.
B)  agar inhibits mold growth.
C)  agar provides a solid surface for bacterial growth.
D)  agar prevents contamination.E) B) and C)
F) C) and D)

Question 6

A differential interference contrast microscope uses dyes to give colored,three-dimensional images.

Accepted Answer

A) True 
 B)False

Question 7

The bending of light rays as they pass from one medium to another is called refraction.

Accepted Answer

A) True 
 B)False

Question 8

The real image is the reverse of the actual specimen.

Accepted Answer

A) True 
 B)False  False

Question 9

Which type of microscope is the most widely used and shows cells against a bright background?

Accepted Answer

A)  bright-field
B)  dark-field
C)  phase-contrast
D)  fluorescence
E)  electronF) All of the above
G) A) and B) A

Question 10

A microbiologist makes a fixed smear of bacterial cells and stains them with Loeffler's methylene blue.All the cells appear blue under the oil lens.This is an example of

Accepted Answer

A)  negative staining.
B)  using an acidic dye.
C)  simple staining.
D)  using the acid-fast stain.
E)  capsule staining.F) A) and D)
G) A) and C)

Question 11

The wavelength of light used and the numerical aperture governs

Accepted Answer

A)  illumination.
B)  resolution.
C)  magnification.
D)  size of the field.E) B) and D)
F) None of the above

Question 12

Which of the following is not an inoculating tool?

Accepted Answer

A)  Petri dish
B)  loop
C)  needle
D)  pipette
E)  swabF) B) and C)
G) C) and D)

Question 13

Which of the following is produced by adding 1% to 5% agar to nutrient broth that is then boiled and cooled?

Accepted Answer

A)  a pure culture
B)  a mixed culture
C)  a solid medium
D)  a liquid medium
E)  a contaminated mediumF) A) and C)
G) B) and C)

Question 14

Fluoresence microscopy uses a different wavelength of light to illuminate a specimen versus other types of microscopes.This results in:

Accepted Answer

A)  more magnification
B)  better resolution
C)  less refraction
D)  more distortion of the object
E)  halos around the objectsF) All of the above
G) D) and E)

Question 15

Bacteria that require special growth factors and complex nutrients are termed

Accepted Answer

A)  aerobic.
B)  anaerobic.
C)  fastidious.
D)  microaerophilic.
E)  autotrophic.F) B) and E)
G) A) and D)

Question 16

For which bacterial genus is mannitol salt agar selective?

Accepted Answer

A)  Salmonella
B)  Streptococcus
C)  Neisseria
D)  Staphylococcus
E)  EscherichiaF) A) and E)
G) A) and B)

Question 17

All of the following are examples of different types of microbiological media except

Accepted Answer

A)  broth.
B)  enriched.
C)  agar.
D)  Petri dish.
E)  semisolid.F) A) and D)
G) A) and E)

Question 18

If a microbiologist is studying a specimen at a total magnification of 950X,what is the magnifying power of the objective lens if the ocular lens is 10X?

Accepted Answer

A)  100X
B)  950X
C)  85X
D)  850X
E)  95XF) B) and C)
G) A) and B) E

Question 19

Mixed cultures are also referred to as contaminated cultures.

Accepted Answer

A) True 
 B)False

Question 20

The correct microbiological term for the tiny sample of specimen that is put into a nutrient medium in order to produce a culture is

Accepted Answer

A)  colony.
B)  inoculum.
C)  streak.
D)  loop.E) C) and D)
F) All of the above

Exam 3: Tools of the Laboratory: Methods for the Culturing of Microscopic Analysis of Microorganisms

Which of the following is essential for the development of discrete,isolated colonies?

Correct AnswerverifiedShow Answer

Which type of microscope bombards a whole,metal-coated specimen with electrons moving back and forth over it?

Correct AnswerverifiedShow Answer

Which type of microscope achieves the greatest resolution and highest magnification?

Correct AnswerverifiedShow Answer

Which of the following magnifies the specimen to produce a real image of the specimen?

Correct AnswerverifiedShow Answer

Agar is an important component of media because

Correct AnswerverifiedShow Answer

A differential interference contrast microscope uses dyes to give colored,three-dimensional images.

Correct AnswerverifiedShow Answer

The bending of light rays as they pass from one medium to another is called refraction.

Correct AnswerverifiedShow Answer

The real image is the reverse of the actual specimen.

Correct AnswerverifiedFalse

Which type of microscope is the most widely used and shows cells against a bright background?

Correct AnswerverifiedA

A microbiologist makes a fixed smear of bacterial cells and stains them with Loeffler's methylene blue.All the cells appear blue under the oil lens.This is an example of

Correct AnswerverifiedShow Answer

The wavelength of light used and the numerical aperture governs

Correct AnswerverifiedShow Answer

Which of the following is not an inoculating tool?

Correct AnswerverifiedShow Answer

Which of the following is produced by adding 1% to 5% agar to nutrient broth that is then boiled and cooled?

Correct AnswerverifiedShow Answer

Fluoresence microscopy uses a different wavelength of light to illuminate a specimen versus other types of microscopes.This results in:

Correct AnswerverifiedShow Answer

Bacteria that require special growth factors and complex nutrients are termed

Correct AnswerverifiedShow Answer

For which bacterial genus is mannitol salt agar selective?

Correct AnswerverifiedShow Answer

All of the following are examples of different types of microbiological media except

Correct AnswerverifiedShow Answer

If a microbiologist is studying a specimen at a total magnification of 950X,what is the magnifying power of the objective lens if the ocular lens is 10X?

Correct AnswerverifiedE

Mixed cultures are also referred to as contaminated cultures.

Correct AnswerverifiedShow Answer

The correct microbiological term for the tiny sample of specimen that is put into a nutrient medium in order to produce a culture is

Correct AnswerverifiedShow Answer

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False

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A

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E

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