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When a virus has a broad host range


A) it does not require close contact to infect a new host
B) it can infect many different cell types
C) it can infect many different species
D) the species that the virus can infect are spread over a large geographic distance,potentially causing pandemics
E) it can infect many cell types or species

F) A) and B)
G) A) and E)

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During HIV reproduction,spike glycoproteins


A) do not enter the cell with the virus
B) are released into the cytoplasm by cellular enzymes
C) undergo a conformational change to inject viral contents into the host cell
D) are assembled into the virus along with reverse transcriptase
E) are embedded in the viral capsid

F) B) and D)
G) A) and B)

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A particle that consists of nucleic acids surrounded by protein and requires a host organism to replicate is


A) a prokaryote
B) a eukaryote
C) a plasmid
D) a virus
E) bacterium

F) A) and E)
G) A) and B)

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How did Davis demonstrate that bacterial cells must be in physical contact in order for genetic exchange to occur?


A) He mixed together two bacteria strains with different nutritional requirements,and then added them to agar plates that lacked their required nutrients.
B) When two bacterial strains with different nutritional requirements were combined in a tube and allowed to grow for several hours,they acquired the ability to grow without the addition of biotin.
C) When two bacterial strains with different nutritional requirements were separated from each other by a selective filter,they did not acquire the ability to grow on agar plates that lacked their required nutrients.
D) Only when enough pressure was applied to force bacteria from one side of a selective filter to another did they acquire the ability to grow without their required nutrients.
E) Nutritional deficient strains only acquired the ability to grow without their required nutrients when the pore size of a selective filter was increased to allow bacteria to pass from one side to the other.

F) A) and D)
G) A) and C)

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What mechanism is used by a bacterial cell to fit its DNA into the nucleoid region?


A) the formation of loop domains
B) supercoiling by topoisomerases
C) tight wrapping around histones to form nucleosomes
D) the formation of loop domains and supercoiling by topoisomerases
E) the formation of loop domains,supercoiling by topoisomerases,and tight wrapping around histones to form nucleosomes

F) All of the above
G) C) and D)

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Plasmids can help bacteria grow faster.

A) True
B) False

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A pro- strain of bacteria,which has not been in contact with any other strains,develops the ability to produce the amino acid proline.What is the most likely mechanism of this mutant "rescue?"


A) addition of the pro+ gene via transduction
B) addition of the pro+ gene during conjugation
C) removal of the pro- gene during excision
D) addition of the pro+ gene via transformation
E) correction of the pro- gene by reverse transcriptase

F) D) and E)
G) A) and D)

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Plasmids replicate independently of the bacterial chromosome

A) True
B) False

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The figure below represents a lineage of a bacterial strain.Upon further studies of this system,you learn that the phage that infects the blue smile bacteria has a very small host range,and cannot infect the red smile bacteria.In light of this new information,determine what is the most likely mechanism that induced a change in the phenotype of this lineage. The figure below represents a lineage of a bacterial strain.Upon further studies of this system,you learn that the phage that infects the blue smile bacteria has a very small host range,and cannot infect the red smile bacteria.In light of this new information,determine what is the most likely mechanism that induced a change in the phenotype of this lineage.   A) Random mutation B) Conjugation C) Transformation D) Transduction E) Lysogeny


A) Random mutation
B) Conjugation
C) Transformation
D) Transduction
E) Lysogeny

F) A) and B)
G) None of the above

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Bacterial cells always contain one copy of a circular chromosome.

A) True
B) False

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How is binary fission similar to the mitosis/cytokinesis?


A) both are modes of sexual reproduction
B) both occur only in eukaryotes
C) both occur only in prokaryotes
D) both involve replication of DNA and separation of DNA equally into two daughter cells
E) both give rise to offspring that are genetically different from the parent

F) B) and D)
G) B) and C)

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Lederberg and Tatum's landmark experiment showed that gene transfer could occur between bacteria,by mixing two strains of bacteria.One strain of bacteria could not synthesize methionine or biotin,but was capable of synthesizing threonine and proline.The other strain was able to synthesize methionine and biotin,but was not capable of synthesizing threonine and proline.Only when these strains were mixed together would they grow on agar that lacked these compounds.What negative control would Lederberg and Tatum have used to ensure that their experiments were accurate?


A) A bacteria that could synthesize methionine,biotin,threonine,and proline.
B) A bacteria that could not synthesize methionine,biotin,threonine,or proline.
C) An agar media that contained methionine,biotin,threonine,and proline.
D) An agar medium that lacked methionine,biotin,threonin,proline,and other nutrients that all bacteria require to grow (e.g.glucose) .
E) A plate that contained no bacteria

F) All of the above
G) A) and D)

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Bacterial plasmids


A) are essential for growth
B) can provide genes that allow the bacteria to grow and thrive in the presence of potential toxins
C) are essential for cellular respiration
D) are artificially created by humans and are present in bacteria only because humans put them there
E) are essential for chromosome replication and binary fission

F) A) and D)
G) A) and E)

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Viral genomes must always be excised from the bacterial chromosome before viral components can be produced.

A) True
B) False

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You analyze two daughter cells of Bacteria A immediately after binary fission.Which of the following is ACCURATE about the daughter cells?


A) Each will have about half the number of plasmids as the mother cell.
B) They are members of the same colony.
C) They have genetically identical chromosomes.
D) They are members of the same colony and have genetically identical chromosomes.
E) They are members of the same colony,will have genetically identical chromosomes,and each will have about half the number of plasmids as the mother cell.

F) All of the above
G) A) and E)

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Which of the following is not a part of the general viral reproductive cycle?


A) viral recognition of host surface proteins
B) genome entry into the cell and immediate expression of some viral genes
C) viral proteins are synthesized
D) reverse transcriptase converts RNA to DNA
E) fully assembled viruses are released

F) A) and D)
G) A) and E)

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A __________ bacterial cell is able to take up DNA from the environment.


A) competent
B) virulent
C) fertile
D) high frequency
E) transduced

F) A) and E)
G) D) and E)

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Lederberg and Tatum's landmark experiment showed that gene transfer could occur between bacteria,by mixing two strains of bacteria.One strain of bacteria could not synthesize methionine or biotin,but was capable of synthesizing threonine and proline.The other strain was able to synthesize methionine and biotin,but was not capable of synthesizing threonine and proline.Only when these strains were mixed together would they grow on agar that lacked these compounds.What positive control would Lederberg and Tatum have used to ensure that their experiments were accurate?


A) A bacteria that was not cable of synthesizing methionine,biotin,threonin or proline.
B) A bacteria that was capable of synthesizing methionine,biotin,threonin and proline.
C) An agar medium that contained methionine,biotin,threonin and proline.
D) An agar medium that lacked methionine,biotin,threonin,proline,and other nutrients that all bacteria require to grow (e.g.glucose) .
E) No positive control would be used in this experiment because there are already two different strains of bacteria being tested.

F) B) and C)
G) D) and E)

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You are working in the field with your favorite bacteria species,Bacterium BB.You carry out some experiments with Bacterium BB,and find that it can now degrade toluene and it is pathogenic! What happened?


A) Bacterium BB acquired degradative and virulence plasmids by horizontal gene transfer
B) Bacterium BB picked up fertility and Col-plasmids via conjugation
C) Bacterium BB was exposed to a bacteriophage which is now in its lytic phase
D) Bacterium BB was transformed with a resistance plasmid and a digestive plasmid
E) Bacterium BB sexually reproduced with another strain of bacteria that had both toluene-degrading gene and virulence gene

F) B) and E)
G) B) and C)

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The figure below represents a lineage of a bacterial strain.Study the figure and determine what is the most likely mechanism that induced a change in the phenotype of this lineage. The figure below represents a lineage of a bacterial strain.Study the figure and determine what is the most likely mechanism that induced a change in the phenotype of this lineage.   A) Random mutation B) Conjugation C) Transduction D) Transformation E) Lysogeny


A) Random mutation
B) Conjugation
C) Transduction
D) Transformation
E) Lysogeny

F) D) and E)
G) C) and D)

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