Question 1

There are five families of mature transcripts produced from the major late gene of adenovirus.Which of the following is the MAJOR determinant for which family is produced from the primary transcript?

Accepted Answer

A)  The location of the polyadenylation site.
B)  The location of the 5' splice site.
C)  The location of the 3' splice site.
D)  The location of the transcription start site.
E)  The location of the start codon.F) None of the above
G) A) and B)

Question 2

The CR3 region,found on the13S version of the E1A protein,is involved in activation of transcription by binding to which of the following proteins?

Accepted Answer

A)  CBP
B)  TBP
C)  ATF
D)  CBP and TBP
E)  ATF and TBPF) A) and D)
G) A) and C)

Question 3

The proteins produced from which of the following genes from adenovirus serves to suppress apoptosis in infected cells?

Accepted Answer

A)  E1A
B)  E1B
C)  E2
D)  E3
E)  Major late geneF) All of the above
G) B) and E)

Question 4

Adenovirus has a linear double-stranded DNA genome.Describe how adenovirus DNA is replicated so that the "end problem" is solved.

Accepted Answer

Adenovirus has an unusual solution to so...

Question 5

Because adenovirus produces all of the enzymes it needs for DNA replication,it is not dependent on the host cell entering the S phase of the cell cycle.

Accepted Answer

A) True 
 B)False

Question 6

The tripartite leader found on all of the transcripts produced from the major late gene of adenovirus carries out which of the following functions?

Accepted Answer

A)  It makes the mRNA more stable.
B)  It binds to the eIF4F translation factor.
C)  It shunts the ribosome to the correct AUG on the mRNA.
D)  It assists with transport of the mRNA out of the nucleus.
E)  It improves the efficiency of mRNA splicing.F) A) and D)
G) A) and B)

Question 7

During infection with adenovirus which of the following events activates the production of the major late transcripts?

Accepted Answer

A)  Entry of the viral genome into the nucleus.
B)  Replication of the viral genome.
C)  Transcription of the E1A gene.
D)  Transcription of the E1B gene.
E)  Transcription of the E2 gene.F) A) and E)
G) B) and D)

Question 8

What is the function of the VA RNAs produced by adenovirus?

Accepted Answer

A)  Inhibition of the cellular RNA polymerase III.
B)  Inhibition of the interferon induced PKR kinase.
C)  Stimulation of translation of viral mRNAs.
D)  Regulation of mRNA stability.
E)  Inhibition of the adaptive immune system.F) A) and D)
G) D) and E)

Question 9

Which component of the adenovirus virion binds to the host cell receptor?

Accepted Answer

A)  Knob on the fiber protein.
B)  Penton base.
C)  Hexons.
D)  Minor capsid proteins.
E)  Both "a" and "b" can bind to different receptors.F) B) and E)
G) None of the above

Question 10

Transcription from the major late promoter of adenovirus produces a primary transcript that is over half the length of the virus genome.

Accepted Answer

A) True 
 B)False

Question 11

All of the following are involved in determining the type of mRNAs produced during adenovirus infection EXCEPT…..

Accepted Answer

A)  Transcription initiation by RNA polymerase II.
B)  Location of the transcriptional promoters.
C)  Location of the start codons.
D)  Alternative mRNA splicing.
E)  Alternative polyadenylation sites.F) A) and D)
G) C) and E) C

Question 12

The unique 5' cap and 3' poly(A)tail in the late transcripts of adenovirus allow the ribosome to begin translation at the correct start codon.

Accepted Answer

A) True 
 B)False

Question 13

The E1A protein from adenovirus can act as an oncogene by inducing tumors in experimental animals.

Accepted Answer

A) True 
 B)False

Question 14

The E2 gene encodes all of the following proteins involved in replicating the adenovirus genome EXCEPT:

Accepted Answer

A)  DNA polymerase
B)  Preterminal protein.
C)  Single-stranded DNA binding protein.
D)  Ligase
E)  All of these proteins are encoded by the E2 gene.F) A) and B)
G) A) and C)

Question 15

During DNA replication of the adenovirus genome,the virus only uses continuous synthesis,which means that it does not produce Okazaki fragments.

Accepted Answer

A) True 
 B)False

Question 16

If the E1A gene is transfected into cultured cells,it causes an abortive transformation and the cells die.If the E1B gene is included with E1A,then cells become fully transformed and survive.What critical function does the E1B gene provide?

Accepted Answer

A)  The E1B proteins suppress apoptosis.
B)  The E1B proteins prevent the degradation of the p53 protein.
C)  The E1B proteins assist with transport of mRNAs out of the nucleus.
D)  The E1B proteins bind to Rb and cause the cell to enter S phase.
E)  The E1B proteins activate transcription from p53 inducible promoters.F) C) and D)
G) A) and E) A

Question 17

Adenovirus virions are composed of which of the following components?

Accepted Answer

A)  Hexons composed of trimers of the hexon protein.
B)  Penton bases composed of five copies of the penton protein.
C)  Penton fibers composed of trimers of polypeptide IV.
D)  Basic polypeptides bound to the viral DNA.
E)  All of the above are correct.F) B) and E)
G) B) and C) E

Question 18

Which of the following was NOT studied or discovered in adenoviruses?

Accepted Answer

A)  mRNA splicing
B)  Transcription initiation by RNA polymerase II
C)  Regulation of protein translation.
D)  Proteins involved in regulating transcription.
E)  Use of viruses as gene therapy vectors.F) D) and E)
G) C) and D)

Question 19

The E1A protein from adenovirus indirectly stabilizes the cellular p53 protein.Why is this potentially a problem for the virus?

Accepted Answer

A)  This will activate the adaptive immune response against virally infected cells.
B)  This will initiate the RNAi pathway,which leads to degradation of viral RNA.
C)  This will induce the apoptosis pathway,which leads to cell death.
D)  The virus requires active p53 protein to activate viral genes.
E)  The virus does not use p53,so this is not a problem at all.F) A) and C)
G) A) and B)

Question 20

During the cell cycle in an uninfected cell,the cellular protein Rb represses S phase genes by doing which of the following?

Accepted Answer

A)  Localizes histone deacetylase to E2F inducible genes.
B)  Binds to the adenovirus E1A protein and releases E2F.
C)  Prevents binding of the cellular RNA polymerase II to E2F inducible genes.
D)  Binds and phosphorylates the cyclin dependent kinase.
E)  Prevents the binding of E2F to the promoters of S phase genes.F) B) and C)
G) A) and B)

Exam 23: Adenoviruses

There are five families of mature transcripts produced from the major late gene of adenovirus.Which of the following is the MAJOR determinant for which family is produced from the primary transcript?

Correct AnswerverifiedShow Answer

The CR3 region,found on the13S version of the E1A protein,is involved in activation of transcription by binding to which of the following proteins?

Correct AnswerverifiedShow Answer

The proteins produced from which of the following genes from adenovirus serves to suppress apoptosis in infected cells?

Correct AnswerverifiedShow Answer

Adenovirus has a linear double-stranded DNA genome.Describe how adenovirus DNA is replicated so that the "end problem" is solved.

Correct AnswerverifiedAdenovirus has an unusual solution to so...View AnswerShow Answer

Because adenovirus produces all of the enzymes it needs for DNA replication,it is not dependent on the host cell entering the S phase of the cell cycle.

Correct AnswerverifiedShow Answer

The tripartite leader found on all of the transcripts produced from the major late gene of adenovirus carries out which of the following functions?

Correct AnswerverifiedShow Answer

During infection with adenovirus which of the following events activates the production of the major late transcripts?

Correct AnswerverifiedShow Answer

What is the function of the VA RNAs produced by adenovirus?

Correct AnswerverifiedShow Answer

Which component of the adenovirus virion binds to the host cell receptor?

Correct AnswerverifiedShow Answer

Transcription from the major late promoter of adenovirus produces a primary transcript that is over half the length of the virus genome.

Correct AnswerverifiedShow Answer

All of the following are involved in determining the type of mRNAs produced during adenovirus infection EXCEPT…..

Correct AnswerverifiedC

The unique 5' cap and 3' poly(A)tail in the late transcripts of adenovirus allow the ribosome to begin translation at the correct start codon.

Correct AnswerverifiedShow Answer

The E1A protein from adenovirus can act as an oncogene by inducing tumors in experimental animals.

Correct AnswerverifiedShow Answer

The E2 gene encodes all of the following proteins involved in replicating the adenovirus genome EXCEPT:

Correct AnswerverifiedShow Answer

During DNA replication of the adenovirus genome,the virus only uses continuous synthesis,which means that it does not produce Okazaki fragments.

Correct AnswerverifiedShow Answer

If the E1A gene is transfected into cultured cells,it causes an abortive transformation and the cells die.If the E1B gene is included with E1A,then cells become fully transformed and survive.What critical function does the E1B gene provide?

Correct AnswerverifiedA

Adenovirus virions are composed of which of the following components?

Correct AnswerverifiedE

Which of the following was NOT studied or discovered in adenoviruses?

Correct AnswerverifiedShow Answer

The E1A protein from adenovirus indirectly stabilizes the cellular p53 protein.Why is this potentially a problem for the virus?

Correct AnswerverifiedShow Answer

During the cell cycle in an uninfected cell,the cellular protein Rb represses S phase genes by doing which of the following?

Correct AnswerverifiedShow Answer

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Adenovirus has an unusual solution to so...
View Answer

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C

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A

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E

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